Green Global
Foundation   Journal
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IN VITRO PLANT REGENERATION OF LENTIL (Lens culinaris Medik.) USING LEAF AND NODAL EXPLANTS (Pages : 1 - 7)

The investigation was carried out in the Tissue Culture Laboratory of the Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh during November, 2009 to April, 2010 with a view to study in vitro regeneration of Lentil (Lens culinaris Medik.). For this purpose, three varieties of Lentil viz. BARI Masur-3, BARI Masur-4 and BARI Masur-5 & different concentrations and combinations of hormones (2, 4-D, NAA, BAP, Kn and IAA) were used to assess regeneration ability by using two explants (leaf disc and nodal segments). Leaf disc and nodal segments of the three genotypes of Lentil were cultured on MS medium with different concentrations and combinations of growth regulators. Among the three varieties, BARI Masur-4 showed early callusing (17.67 days) with maximum rate of callus induction (83.33%) incase of leaf disc culture and also showed highest percentage of callus induction (80.55%) with minimum days (17.33 days) with nodal segment as explant. Early and maximum rate of callusing appeared in (MS + 1.5 mg/L 2, 4-D + 0.5 mg/L BAP) and (MS + 2 mg/L 2, 4-D + 0.5 mg/L NAA) from leaf disc and nodal segment respectively. BARI Masur-4 had the highest percentage of shoot regeneration from leaf disc (69.44%) and nodal segment (66.66%) explants. Early and maximum rate of regeneration was found in (MS + 2.0 mg/L Kn + 0.2 mg/L NAA) and (MS medium + 0.5 mg/L BAP + 0.25 mg/L Kn). The highest number of roots per shoot was counted in BARI Masur-4 (47.23%), in (MS medium containing 10mg/L IAA) and (MS medium containing 15mg/L IBA) considering leaf disc and nodal segment as explant. Considering the overall performance, BARI Masur-4 appeared to be the best genotype for callus formation, shoot regeneration and root formation. Download