Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

IN VITRO PROPAGATION OF EMBRYONIC EXPLANTS OF SATKARA (Citrus macroptera Montrouz) (Pages : 19 - 24)

Citrus macropter is a wild or semi wild Citrus species grow well in hill-forest. The present investigation was conducted to study the effects of auxin and cytokinin on callus induction from embryonic explants of Citrus macroptera Mont. The embryonic explants produced semi-hard greenish and creamy-white callus and adventitious buds within 4-5 week of culture. The highest percentage of callus is produced in media containing 2.0 mgl -1 2,4-D+1.5 mgl -1 KIN were used. The rate of adventitious shoot regeneration from callus was also influenced different concentration of BA in MS medium. The highest shoot regeneration rate was observed in embryogenic callus on MS media supplemented with 1.5 mgl -1 BA. Best rooting achieved within 28 days after the isolated adventitious shoots were transferred to a rooting media containing 1.0 mgl -1 IBA. In vitro cultured plantlets transfer to pot soil and successfully acclimatized with natural condition. Download