Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

IN VITRO SEEDS GERMINATION AND PLANTLETS DEVELOPMENT OF SILVIUS APPENDICULATUS (Orchidaceae) (Pages : 1 - 5)

Bangladesh is rich in orchids, with 159 species and two varieties under 63 genera (Huda et al.1999). These species are distributed mainly in the hilly areas of greater Sylhet, Chittagong and Mymensingh districts. Seeds of S. appendiculatus were germinated successfully and grew well in the in vitro culture. The best medium were observed for germinating the S. appendiculatus seeds, viz. Knudson-C (KnC) basal medium supplemented with 30% (v/v) maturity coconut water/milk (CW). All the seeds were observed those overall germination, protocorms formation and seedling development (about 100%). The best medium for growing and development of seedlings to become fully expanded plantlets was determined half strength of Murashige and Skoog (1/2MS) medium supplemented with 40% (v/v) CW. The plantlets of S. appendiculatus were resulted from these research approximately more than 2000 ndividuals. The fully expanded seedlings were transferred to perforated plastic pots and grow in the screen house. iDownload