USE OF Trichoderma IN BIOLOGICAL CONTROL OF FOOT AND ROOT ROT OF LENTIL (Lens culinaris Medik) (Pages : 29 - 35)


A series of experiments were conducted to assess the effect of 14 isolates of Trichoderma spp. (Trichoderma harzianum and T. viride) for control of foot and root rot of lentil (Lens culinaris Medik) caused by Fusarium oxysporum Schlecht. The pathogenecity of 12 isolates of F. oxysporum and the mass production of an isolate of T. harzianum on 25 substrates were also studied. The isolates of Trichoderma spp. and F. oxysporum were collected from different locations of Bangladesh. Trichoderma isolates inhibited the growth of F. oxysporum from 45.87 to 92.07 % at 7 days after inoculation on agar plates. The isolate TG-2 of T. harzianum showed the highest inhibition of the pathogen in field condition. The lowest foot and root rot incidence (6.9%), highest seed germination (82.08%), maximum plant stand (93.12%) and the highest seed yield (3726.67 kg ha-1 ) were recorded in plots where the isolate TG-2 was applied. In pathogenecity test, the highest foot and root rot incidence and the lowest plant stand of lentil were recorded in the pots where soil inoculation with the isolate FBg-1 of F. oxysporum was done. The highest colony forming unit (4.04 x 106 /g) of T. harzianum (TG-2) was recorded in chickpea bran. The study concluded that the isolate TG-2 of T. harzianum can be used to control foot and root rot disease of lentil in Bangladesh and chickpea bran is a useful substrate for mass production of that isolate for soil application. Download

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