Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

MOLECULAR CHARACTERIZATION OF EGGPLANT CROSSES BY USING RAPD ANALYSIS (Pages : 22 - 28)

Random amplified polymorphic DNA (RAPD) technique was used to determine the genetic variation and relationships among three parents (BAU Begun-1, Dohazari G and Laffa S) and five F5 offsprings (BAU Begun-1 x Laffa S and BAU Begun-1 x Dohazari G) of eggplant. Amplification with 3 decamer primers generated 28 and 31 bands in Laffa S x BAU Begun-1 and Dohazari G x BAU Begun-1 respectively from which 16 (57.14%) and 18 (58.06%) were polymorphic, respectively. The co-efficient of gene differentiation (Gst) was 0.4534 reflecting the existence of high level of genetic variations among the genotypes. The dendrogram (UPGMA) constructed from Nei’s genetic distance produced 2 main clusters of the parents and F5 offsprings. Higher genetic distance (0.5256) and lower genetic identity (0.5799) was observed between the genotype combinations. Genetic variation and relatedness to disease reaction as assessed using RAPD markers could be potential sources for the development of Phomopsis vexans resistant variety which is one of the major biotic stresses in eggplant in Bangladesh. Download