Green Global
Foundation   Journal
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IN VITRO DIRECT AND INDIRECT PLANTLETS REGENERATION FROM NODAL SEGMENT OF Boerhaavia repens L. (Pages : 5 - 10)

An efficient protocol for in vitro propagation of Boerhaavia repens L. was established using nodal segment. The highest direct shoot regeneration from nodal segment was noted (94%) in the MS medium having 2.0 mg/l BAP+0.2 mg/l NAA. The mean number of shoots per culture (12.51) and mean length of shoot per culture (5.10 cm) were also highest in the same medium. Callus induction and plantlet regeneration were observed using different concentrations and combinations of growth regulators. The highest frequency (100%) of organogenic callus was observed in the MS medium containing 1.0 mg/l NAA+1.0 mg/l BAP. Again, the highest (91%) shoot was produced in the MS medium in the combination with 2.0 mg/l BAP+1.5 mg/l Kn+0.5 mg/l NAA was proved to be most suitable in shoot formation from callus. The elongated shoots developed maximum (100%) roots on a half strength MS medium containing 1mg/l IBA. The well rooted plantlets were successfully established in natural condition, where the survival rate of plantlets was 80%. Download