Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

MICROPROPAGATION OF STRAWBERRY (Fragaria x ananassa Duch.) (Pages : 36 - 41)

The experiment was conducted from March 02 to September 25, 2010 at Plant Tissue Culture Laboratory of Genetic Engineering and Biotechnology Department, Jessore Science and Technology University, Jessore, Bangladesh to clarify the effective concentration of growth regulators for micropropagation of strawberry. Response of medial additives like adenine sulfate (120mgL-1 ) and coconut water (150 mgL-1 ) were also investigated. The concentration of cytokinin-auxin was ranged from 1.0-3.0 mgL-1 and 0.1-0.3 mgL-1 respectively. Addition of adenine sulfate, gibberellic acid and coconut water was insignificant than combine concentration of cytokinin and auxin regarding usable shoots. But shoot length was the highest (2.4 cm) which is significantly different than that of gibberellic acid and coconut water. Maximum number of nods was also observed in the presence of adenine sulfate. For rooting the shoots were transferred to MS medium with four different combination of IBA (0.1, 0.2, 0.5, 1.0) mgL-1 and IAA (0.1, 0.2, 0.5, 1.0) mgL-1 individually. Low concentration of IAA produces maximum number of roots which was 16.5 and significantly different than that IBA or IBA IAA combination. But the highest root length was found in the combined treatment of IBA and IAA (0.1+0.1) mgL-1 . Download