Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

IN VITRO PLANT REGENERATION FROM COTYLEDON AND INTERNODES DERIVED CALLUS IN WATERMELON (Citrulus lanatus Thumb.) (Pages : 25 - 29)

A study was carried out to observe the callus induction and subsequent regeneration potentiality of watermelon (Citrullus lanatus Thumb.) from cotyledon and internodes of this plant. Greenish compact callus was achieved from cotyledon on MS medium supplemented with 1 mg/l 2, 4-D within one week of inoculation. The callus produced large number of shoots when cultured on MS medium with1.0 mg/l BAP+0.2 mg/l NAA. Rooting rate was 100% when shoots from second subculture were cultured in medium with 1.0 mg/1 IBA and the shoots were more prone to rooting by increasing subcultures. In vitro grown plantlets with well root system were successfully established in natural condition through successive phases of acclimatization. The regenerated plantlets were healthy, uniform and identical to donor plants and survival percentage was 80%. Download