Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

STUDY ON DIFFERENTIATION OF WHEAT VARIETIES THROUGH MORPHOLOGICAL AND MOLECULAR APPROACHES (Pages : 29 - 34)

Genetic variation within species has been assessed by many methods and from several perspectives. In present study, both RAPD markers and morphology were successfully used to differentiate four cultivars of wheat named Bijoy, Prodip, Sourav and Shatabdi. Four distinct morphological characters were identified to differentiate four varieties. In lower glume beak length, at physiological maturity stage, distinctness was observed. In case of Bijoy variety, it was almost rudiment (1-2mm), but in Prodip, its length was the highest (15-18mm), medium length (12-15mm) was observed in Shatabdi and Sourav showed small (8-l0mm) glume beak length. In Zadok growth stage 25-29, a distinct difference was observed in four wheat varieties. Growth habit of Sourav was erect, Shatabdi semi-erect and Prodip intermediate. Heading days at Zadok growth stage were 68 for Sourav, 71 for Shatabdi, 63 for Bijoy and 60 for Prodip. Culm glaucosity was strong in Sourav, weak in Shatabdi and Prodip and medium in Bijoy. Two primers (OPA-02 and OPB-O1) yielded comparatively higher number of amplification products among the eleven primers with high intensity, minimal smearing and good resolutions with clear bands. All of them (100%) were considered as polymorphic and no monomorphic band was found. The primer OPA-02 produced 16 bands and the other primer OPB-O1 generated 17 bands respectively. The result of RAPD marker was more distinct than the morphological marker for the differentiation of wheat varieties. Phenotypic characters were influenced by the environment but in case of molecular marker the reproducibility of RAPD markers were the same in the same reaction conditions. Download