Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

AGROBACTERIUM-MEDIATED GENE TRANSFER IN POTATO FOR ABIOTIC STRESS TOLERANCE (Pages : 1 - 7)

The experiment was carried out to standardize the callus induction potentiality and transformation ability of leaf and callus explants of potato cv. Granola during the period of January to June 2009 in the Department of Biotechnology, Bangladesh Agricultural University, Mymensingh. Callus induction ability of leaf explants was observed in response to different concentrations and combinations of growth regulators supplemented to MS medium. The highest number of calli per vial (5.67), percentage of callus induction (94.44%) and callus weight (2.66 g) were observed in MS medium containing 3.0 mgL-1 NAA and 2.5 mgL-1 BAP. No callus was observed in medium without growth regulators. Leaf discs and callus explants of potato cv. Granola were inoculated with Agrobaterium tumefaciens strain LB4404 carrying CIPK (abiotic stress tolerant) sense gene and a binary vector pBI121, which contains GUS (reporter gene) and nptII (kanamycin resistance) gene. The transformation work was performed with different infection times and co-cultivation periods. The leaf discs and callus of potato cv. Granola were found GUS positive after inoculation and selection. The highest GUS positive response (83.33%) was recorded from the leaf disc when inoculated for 7 minutes and co-cultured for 5 days. The highest GUS positive response (88.89 %) was observed for 3 minutes inoculation time and 5 days co-cultivation periods in case of callus of potato cv. Granola. These results of GUS histochemical assay indicated that, abiotic stress tolerant CIPK sense gene was transferred in potato cv. Granola. Download