Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

MICROPROPAGATION OF SUGARCANE THROUGH LEAF SHEATH CULTURE (Pages : 13 - 15)

An experiment was conducted to develop an efficient protocol for micropropagation of sugarcane using leaf sheath culture method of three sugarcane varieties viz. Isd16, Isd36 and Isd37 in the germplasm laboratory of breeding division, Bangladesh Sugarcane Research Institute (BSRI), Ishurdi, Pabna. Leaf sheath explants were used for callus formation followed by shoot initiation. Basic medium Murashige and Skooge (MS) supplemented with different concentration of auxin (NAA and 2, 4-D) were used to observe their effects on callus formation. Best results were observed from the varieties Isd36 and Isd37 at 4.0 mg/l of 2, 4-D and the variety Isd16 at 3.0 mg/l of 2, 4-D for callus induction. Different combinations of BAP with NAA or IBA supplemented with MS medium were used for shoot initiation and multiplication. 1.0 mg/l BAP + 0.5 mg/l NAA showed the best result for induction and multiplication of shoot. MS medium supplemented with different concentrations of auxin (NAA and IBA) were used alone for in vitro root formation from proliferated shoots. NAA showed better performance than IBA for root initiation. Best result of root formation was observed on MS medium supplemented with 5.0 mg/l of NAA. The variety Isd37 produced the highest number of roots (13.47) at 5.0 mg/l of NAA. The plantlets were successfully transferred to soil with eighty to ninety percent survivability at normal temperature with 85% humidity. Download