Papers

EFFECTS OF GROWTH REGULATORS, EXPLANT AND GENOTYPE ON SHOOT REGENERATION IN TOMATO (Lycopersicon esculentum c.v. Omdurman) (Pages : 7 - 13)

SHADIN ISHAG, MAGDOLEEN G. OSMAN AND MUTASIM M. KHALAFALLA

Development of protocols for in vitro regeneration can provide new advances for the production of stress tolerant cultivars. The present investigation was developed at the laboratory of plant tissue culture, Commission for Biotechnology and Genetic Engineering, Khartoum, Sudan, during the period of February 2008 to July 2009. Shoot tip and cotyledon explants isolated from 8-10-day-old seedlings were aseptically cultured on a Murashige Skoog (MS) medium supplemented with various concentrations and combinations of growth regulators under defined environmental conditions. Benzyladenine (BA), kintin (Kin) and 2-isopentenyladenine (2ip) at various concentrations (0.5 – 5.0 mg/l) individually or in combination with naphthaleneacetic acid (NAA; 0.5 – 1.0 mg/l) were tested. . Both explant and growth regulators type and concentrations influenced shoot proliferation. Shoot tip was more efficient than cotyledon explant. Kin proved to be more effective than BA. The best response (2.0 shoots/explant), however was obtained when shoot tip cultured on MS medium supplemented with kin at 4.0mg L-1. The efficiency of both BA and Kin for multiple shoot induction was negatively affected when combined with NAA. Shoots produced in vitro were rooted on half-strength agar-solidified MS basal medium or without growth regulator or 0.5 mgl?1 naphthalenacetic acid (NAA) prior to acclimatization and transfer to green house conditions. Regenerated plants grew in soil to maturity and were found to be fertile and morphological true-to-type. This procedure was found to be applicable to other five cultivars of tomato. Thus, it can be advantageously used in combination with conventional breeding techniques for the development of cultivars resistant to biotic and abiotic stresses.Download


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