Green Global
Foundation Journal
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IN VITRO MORPHOGENESIS AND PLANT REGENERATION OF BAMBOOS (Oxytenanthera abyssinica A. Rich. Munro) (Pages : 72 - 79)
EIMAN E. E. DIAB AND SYADAT E. MOHAMEDWith aim of large production of plant material, a protocol for micropropagation of Oxytenanthera abyssinica was developed at the laboratory of Plant Tissue Culture, Commission for Biotechnology and Genetic Engineering, Khartoum, Sudan, during November 2006 to April 2008. Nodal explant from 12 months seedlings age was cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of benzyl adenine (BA) in combination with Naphthalene Acetic Acid (NAA). The highest shoot multiplication (4.4/shoot/explant) was achieved on six weeks on MS supplemented with 5.0 mg L-1 BA and 0.2 mg L-1 NAA. Proliferation media in liquid status shows better performance than solid media. For elongation, in vitro produced shoots were cultured on MS basal media (full, half, quarter) strength, MS full strength achieved the high shoot length (7.6) cm. When the propagated shoots were transferred to a rooting medium (full, half, quarter strength) MS, supplemented with (0, 0.5, 1.0, 2.0, 4.0, 8.0 mg L-1 ) IBA concentrations, the highest rooting percentage (70%) was achieved in full strength MS media supplemented with 8.0 mg L-1 IBA. Plants were successfully established in soil and adapted to greenhouse conditions and field. The protocols developed in this work provide a basis to achieve massive propagation of Oxytenanthera abyssinica by in vitro culture of nodal segments. Download