Green Global
Foundation Journal
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Papers
STUDY ON IN VITRO PROPAGATION THROUGH MULTIPLE SHOOT PROLIFERATION IN WOOD APPLE (Aegle marmelos L.) (Pages : 16 - 20)
R. DAS, M. F. HASAN, M. S. RAHMAN, M. H. RASHID AND M. RAHMANThe experiment was carried out at Professor Ali Md. Eunus Laboratory, Department of genetic Engineering and Biotechnology, university of Rajshahi, Bangladesh, during the period of January to December 2007. An efficient protocol was devised for rapid in vitro propagation through multiple shoot induction from cotyledons and shoot tips of wood apple (Aegle marmelos). The highest percentage of multiple shoots was 91.23% obtained in MS medium augmented with 2.0 mgl -1 BAP+0.2 mgl -1 NAA and the maximum number of multiple shoots was 22.7 per culture obtained in MS medium enriched with 2.0 mgl -1 BAP+0.2 mgl -1 NAA within fourteen days of inoculation. The induced shoots were elongated on the same medium. The elongated shoots were separated and cultured for root induction. Rooting of in vitro raised shoots was best induced on half strength MS medium supplemented with 1.0 mgl -1 IBA with highest percentage of shoot regenerating roots (80.42%) with 4 roots per shoot. The well rooted plantlets were acclimatised and successfully established onto the natural condition with 80% survival. Download