Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

IN VITRO RESPONSE OF DIFFERENT EXPLANTS ON CALLUS DEVELOPMENT AND PLANT REGENERATION IN GROUNDNUT (Arachis hypogeae L.) (Pages : 1 - 4)

Different explants namely, cotyledon, epicotyl and hypocotyl were used to study the in vitro plant regeneration of groundnut (Arachis hypogaea L.). The explants of two different genotypes BARI groundnut 5 and BARI groundnut 6 were cultured in MS medium with different concentration of 2,4-D, BAP and NAA. In case of different explants, hypocotyls showed better performances than epicotyls and cotyledon. Among different concentrations, 2,4-D @ 2mg/l was found more suitable for good callus induction. When callus was sub cultured in different concentration of 2,4-D, good callus growth was also observed. MS medium supplemented with different concentrations of BAP produced small shoot bud at different subculture. Maximum number of shoot bud differentiation was observed from 2.5mg/l BAP concentration. Among the different growth hormones, 2,4-D found good for callus induction and BAP was found more suitable for organogenesis compare to NAA.Download