Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

TISSUE CULTURE PROPAGATION OF TROPICAL ORCHID (Phaius tankervilliae) PLANT (Pages : 81 - 85)

Phaius tankervilliae is a famous ornamental plant which is rarely found in Bangladesh. Its beauty and uniqueness made it an important candidate for tissue culture studies especially for regeneration and multiplication purposes. Here we for the very first time report a rapid and reliable method for the regeneration of Phaius tankervilliae in vitro. Axillary buds explants from one year old Phaius tankervilliae plant were cultured on MS (Murashige and skoog's, 1962) medium without any hormone supplementation. Multiplication was carried out by using 1 mg/lit. and 0.1 mg/lit., BAP (6-benzylaminopurine) and NAA (?-naphthalene acetic acid), respectively. For elongation purpose, 0.5 ?M GA3 (gibberellic acid) was used. 0.1 mg/lit. NAA was used to stimulate extensive root development within 1 month. After successful regeneration, the plantlets were acclimatized into the soil. This study is undertaken to perform in vitro clonal propagation and acclimatization of Phaius tankervilliae in order to get hundreds of Phaius tankervilliae plants in the laboratory in comparatively less time. Hence, it would be possible to regenerate Phaius tankervilliae plants in the laboratory and after successful acclimatization.Download