Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

IN VITRO MULTIPLE SHOOT INDUCTION AND PLANT REGENERATION IN ELITE SUDANESE COTTON (Gossypium hirsutum L.) CULTIVAR (BARAC - B - 67) (Pages : 77 - 82)

This study was carried out at department of Plant Cell and Tissue Culture, Commission for Biotechnology and Genetic Engineering, Khartoum, Sudan during August 2006 to June 2008 to develop a protocol for in vitro regeneration for elite Sudanese cotton cultivar. Induction of multiple shoots in medium stable cotton (Gossypium hirsutum L. cv. Barac - B - 67) has been achieved with cotyledonary nodes devoid of cotyledons and apical meristem. The induction of multiple shoots in explants varied with concentration and type of cytokinin as well as seedlings age. Explants from 35-day-old seedlings yielded the maximum number of shoots (2.8 shoots/explant) using Gamborg (B5) basal medium supplemented with 0.1 mg L-1 Benzyl adenine (BA) and kinetin (Kin) 2.5 mg L-1. Elongation of multiple shoots was obtained on halfstrength agar-solidified B5 basal medium without phytohormones. In vitro shoots were rooted on halfstrength agar-solidified B5 basal medium without growth regulator or with 0.1, 0.5 or 1.0 mg L-1 naphthalene acetic acid (NAA). This procedure was rapid, reliable and with high-frequency. Thus, it can be advantageously used in application of modern gene transfer techniques for the improvement of medium staple cotton cultivar (Barac -B -67).Download