Papers

DETECTION AND CHARACTERIZATION OF POSTHARVEST PATHOGENS OF ORANGE COLLECTED FROM MARKET IN NORTH EASTERN PART OF BANGLADESH (Pages : 17 - 21)

J. AHMED, A. MUQIT AND M. ASAD-UD-DOULLAH

This experiment was carried out to identify and characterize the pathogens associated with postharvest loss of orange in fruit shops at Sylhet city, Bangladesh. A survey was conducted to determine the disease prevalence through collecting samples of sweet orange and mandarin orange from 10 fruit shops at various locations. About 7% of the total observed fruits in the market were found damaged due to postharvest fungal infection. Four different fungi namely, Penicillium digitatum, Penicillium italicum, Geotrichum candidum, and Cladosporium cladosporioides were isolated from the diseased and healthy fruit samples from different fruit shops after incubation of 6-7 days at room temperature. Tissue plating method was used to prepare the pure culture of each pathogen and Koch’s postulate was performed to confirm pathogenicity of individual fungi. P. digitatum, P. italicum, G. candidum, and C. cladosporioides were cultured on potato dextrose agar (PDA) media and formed colonies of green, blue, creamy white, and dark black color. Penicillium sp. developed brush like phialides bearing circular conidia in chains, G. candidum produced subglobose or cylindrical conidia and C. cladosporioides developed lemon shaped, dark black conidia on the nutrient media. Each fungus showed similar symptoms in naturally infected fruits as well as artificially inoculated ones. Most of the pathogens took 3-4 fours days to develop respective symptoms and 6-8 days for total damage of the fruit.Download


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