Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

IN VITRO CALLUS DEVELOPMENT AND PLANT REGENERATION FROM IMMATURE COTYLEDONS-THE BEST TECHNOLOGY FOR SUSTAINABLE GROUNDNUT (Arachis hypogaea L.) PRODUCTION IN THE MODERN ERA (Pages : 26 - 37)

Groundnut (Arachis hypogaea L.) is an important oilseed and protein-rich crop in the world. Plant regeneration system from explants through the application of the biotechnological approach is the best technology in the modern era. Therefore, callus induction and organogenesis from different groundnut explants were studied on MS medium supplemented with different concentrations and combinations of 2,4-D, BAP, Kn, IBA and NAA hormones. Different types of explants viz., cotyledon, epicotyl and hypocotyl have been used by various researchers worldwide to study in vitro plant regeneration but in our study, we used immature cotyledon. Four groundnut genotypes such as GC(1)-35-1, GC(1)-4, GC(1)-3-2-1 and Zhingabadam were used as plant materials. The experiment was conducted in the tissue culture laboratory of the Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202 during the periods of 2010 to 2012. Our results demonstrated a wide range of variations in callus induction under different hormonal concentrations and combinations. Among the chemicals, applications of 2,4-D was found as the best hormone for callus induction in alone or in combination with kinetin. The maximum (75%) proliferated callus development was appeared on in combination of MS + 1.5 mg/L 2,4-D + 0.5 mg/L BAP. The highest number of shoot regeneration (61.53%) were observed when MS medium supplemented with 1.5 mg/L BAP + 1.0 mg/L NAA for genotype GC(1)-35-1 while the maximum percentage of roots induction (75%) was observed in combinations of half MS + 0.5 mg/L IBA from previously regenerated shoots. Among the four genotypes, the explants GC(1)-35-1 was successfully established in soil with the highest (75%) rate of survival and it finally yielded with flowering in the earthen pots.Download