Green Global
Foundation   Journal
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STUDY ON THE ANTIOXIDANT ACTIVITY OF DIFFERENT SOLVENT EXTRACTS OF Abroma augusta LEAVES (Pages : 11 - 17)

To evaluate the antioxidant activity of Abroma augusta leaves, the different fractions of methanolic extracts were taken to determine the total phenolic content, total flavonoid content, reducing power capacity and free radical scavenging activity. Among the fractions, the highest phenolic content was found in ethyl acetate fraction (0.466 ± 0.001 mg gallic acid/g of extract). In chloroform (CF), petroleum ether (PEF) and dia-ion resin absorb fractions (DIRAF) the values were found 0.196 ± 0.001, 0.112 ± 0.001 and 0.111 ± 0.001 mg gallic acid/g of extract respectively followed by methanol fraction 0.255 ± 0.001 mg gallic acid/g of extract. The order of total flavonoid content of different fractions were DIRAF (3.06 ± 0.010) > PEF (2.15 ± 0.001) > CF (0.878 ± 0.001) followed by methanol fraction (1.17 ± 0.001) mg catechin/g of extract. The reducing power capacity exhibited the order of EAF (1.363 ± 0.010) > CLF (0.182 ± 0.014) > PEF (0.065 ± 0.007) > DIRAF (0.047 ± 0.004) nm at 100 mg /ml followed by ascorbic acid standard (3.674 ± 0.008) nm at 100 µg/ml concentration. The total antioxidant activity of the extractives showed the order of EAF (1.935 ± 0.007) > PEF (1.776 ± 0.008) > CLF (0.414 ± 0.007) > DIRAF (0.094 ± 0.012) nm at 100 µg/ml. In case of DPPH radical scavenging activity, DIRAF showed the highest (45.71) µg/ml and EAF showed the lowest (191.17) µg/ml with IC50 followed by BHT standard and the order was: BHT > DIRAF > CLF > PEF >EAF.Download