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EFFECT OF COLCHICINE APPLICATION ON IN VITRO REGENERATION OF TEASLE GOURD PLANTLETS (Pages : 45 - 50)

S.S.U. AHMED, M.A. KARIM, M.S. HAQUE AND M.A. ULLAH

To develop in vitro regeneration protocol several explants (Node, internode, leaf, leaf petiole, shoot tip and cotyledon) and growth regulators (BA, NAA, IBA, IAA) were used for callus, shoot and root induction from teasle gourd (Momordica dioeca) plant. Cotyledon was found to be the best explants and BA at 1.0 mgL-1 was found to be the best for callus initiation and shoot regeneration. IBA at 0.3 mgL-1 was the best for root initiation in ½ MS medium with 3% sucrose. Mature calli were soaked in colchicine solution of 0.05% concentration for short duration (1, 2, 4 & 8 hours). Simultaneously, 4 different concentrations of colchicine (10, 20, 30 & 40 mgL-1) at longer durations (3, 6 & 9 days) were used. Low concentration at short duration (10 mgL-1 for 3 days) had high percentage (90%) of survivability. The highest size (4.0 cm) and weight (5.4 g) of calli was found in 10 mgL-1 colchicine treatment for 3 days. Also highest shoot germination from callus (60%), maximum shoot per flask (32), shoot length (3.0 cm), maximum root per plant (24) and length of root (2.8 cm) was found in this treatment. Four hour treatment of colchicine at 0.05% concentration resulted in vigorous vegetative growth. Rooted plantlets of about 4-6 cm height (2-3 nodes) were subsequently transferred to different media for acclimatization. Plants grown in mixture of soil and cowdung at the proportion of 3:1 and showed 100% of survival rate during rainy season.Download


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