Green Global
Foundation   Journal
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CLONING OF cadherin GENE INTO PLANT BINARY VECTOR AND RETRANSFORMATION INTO cry1Ac TRANSGENIC TOBACCO (Pages : 27 - 34)

Cadherin belongs to the families of animal glycoproteins which is responsible for calcium-dependent cell-cell adhesion. Recent literature showed that the cadherin like in mid gut of several insects served as the receptor of the Bt toxin cry1A and the variation of cadherin-like is related to insect resistance to cry1A. The interaction between Bacillus thuringiensis insecticidal crystal protein cry1A and cadherin receptor in Lepidopteran insects induces toxin oligomerization. In this study to prove this hypothesis that Helicoverpa armigera cadherin fragment (HaCad1) containing its toxin binding region was cloned into binary vector and retransformation has done into the cry1Ac plants through Agrobacterium mediated plant transformation. Insect bioassay was carried out to compare the toxicity enhancement between cry1Ac and cry1Ac with HaCad1 transgenic plants. Based on this insect bioassay, retransformed plants were showed equal resistant to the cry1Ac transgenic plants; it may be the reason for protein expression level of transgenic events. Development of more transgenic events with high protein expression level will be proven the hypothesis of enhancement of insecticidal activity of cry1Ac with cadherin.Download