Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

EFFECT OF GROWTH REGULATORS ON MERISTEM CULTURE OF POTATO CV. KUFRI CHIPS SONA (Pages : 22 - 24)

The experiment was conducted at the Biotechnology Laboratory, A. H. Z. Biotech Limited, Vadra, Rajshahi, Bangladesh during December 2010 to April 2011 to find out a suitable growth regulator combination and concentration of GA3 and NAA with Murashige and Skoog (MS) medium for regeneration of potato cv. Kufri Chips Sona. The treatments were different concentration of GA3 (0.15, 0.25, 0.35 & 0.45 mg/l) + NAA (0.01, 0.02, 0.03, & 0.04 mg/l). It was found that the highest (53%) explants survived and produced shoots on the combination of GA3 @ 0.25 mg/l + NAA @ 0.02 mg/l. The highest (8.80 cm) shoots lengths were recorded with combination of GA3 @ 0.25 mg/l + NAA @ 0.02 mg /l. In respect of node number it was observed that the maximum (8.73) number of node was recorded from GA3 @ 0.25 mg/l + NAA @ 0.02 mg /l. The highest root length (9.60 cm) was recorded in the treatment of GA3 @ 0.25 mg/l + NAA @ 0.02 mg /l. The explants treated with GA3 @ 0.25 mg/l + NAA @ 0.02 mg /l gave the highest fresh shoot and root weight (0.738 & 0.350 g, respectively). It is concluded that the combination of GA3 @ 0.25 + NAA @ 0.02 mg/l performed better for shoot and root regeneration and multiplication of potato cv. Kufri Chips Sona.Download