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IN VITRO STUDIES ON THE REACTION OF FUNGI Trichoderma TO DIFFERENT HERBICIDES USED IN TEA PLANTATION (Pages : 27 - 30)

M. S. ISLAM , A. K SAHA, H. Q. M. MOSADDEQUE, M. R. AMIN AND M. M. ISLAM

An experiment was conducted at the Plant Pathology laboratory of Bangladesh Tea Research Institute on September, 2006 paper investigated selected genus of Trichoderma and herbicides aimed at defining the effect of different concentrations on the development of mycelium of the isolates studied and spore germination. Five commonly used herbicides like Glyphosate 41SL (2022.66 PPM), Paraquat 20 W/W (746.66 PPM), Bimaster 240/120AS (1680.00 PPM), Kem- Amin 58SL (2165.33 PPM) and T5= Butachlor 5G (281.25 PPM) were considered as treatments. Concentrations (PPM) were calculated on the basis of their doses of applications recommended by BTRI. Herbicides were added with melted media separately maintaining the said concentrations of each. Trichoderma was isolated from tea soil by soil dilution method and maintained pure culture on PDA media. After preparation of spore suspension, it was plated with herbicides added PDA media. Every 24 hours observations revealed that Bimaster 240/120AS completely inhibited the growth of Trichoderma. Though the fungus structure was seen in the form of spot like sporulation on the PDA treated with Paraquat 20 W/W, Kem- Amin 58SL and Butachlor 5G. But further these could not produce mycelium. Only 21-30% mycelial growth was found in cultured treated with Glyphosate 41SL.Download


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