Papers

ENHANCEMENT OF AGROBACTERIUM-MEDIATED TRANSFORMATION METHOD FOR THE PRODUCTION OF HEME-PROTEIN (FERRITIN PROTEIN) RICH POTATO (Pages : 17 - 22)

E. H. KHOKAN, A. HAYDAR, T. ARA, M. K. ALAM and M. D. SHARMA

The experiment was conducted at plant breeding and gene engineering laboratory, Department of Botany, Rajshahi University, Rajshahi during 2008-09 to establish an Agrobacterium tumefaciens mediated transformation procedure for Solanum tuberosum. For increasing the iron storage in the potato, explants (in vitro and ex vitro leaf and internode) were incubated with Agrobacterium tumefaciens strain LBA4404 bearing Ti based binary pCAMBIA2301 plasmid containing selectable marker neomycin phosphotransferase (nptII) and ferritin gene. Following co-cultivation explants were cultured on selective medium containing 50 mg/l Kanamycin + 50 mg/l Cefotaxime. Kanamycin resistant shoots were induced from explants after 5-6 weeks. Shoot regeneration was achieved after transferring the tissue on to fresh medium of same combination and the shoots were rooted on MS medium containing 50 mg/l kanamycin. Incorporation and expression of the transgenes were confirmed by PCR and RT-PCR analysis. Transgenic potato could be developed with ferritin gene using this protocol. Download


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