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CALLUS INDUCTION AND PLANT REGENERATION FROM SHOOT TIPS OF CHAKUNDA (Cassia obtusifolia L.) (Pages : 6 - 10)

M. F. HASAN, R. DAS, M. S. RAHMAN, M. H. RASHID, M. S. HOSSAIN AND M. RAHMAN

The present investigation was conducted at Professor Ali Md. Eunus Laboratory, Depertment of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, Bangladesh, during the period of April to December 2007. The experiment was carried out to develop an efficient protocol for regeneration of chakunda (Cassia obtusifolia L.) via callus culture from shoot tips, rooting of callus derived shoots and establishment onto the natural condition. Different concentrations and combinations of auxin and cytokinin were used in full strength ofmedium to observe the callus induction, shoot regeneration and root proliferation. The highest percentage of callus induction was 96.6% observed in the medium supplemented with 2.0 mg1-1 2,4-D. The highest number of shoots was 5.0 found in the medium having 2.0 mg1-1 2.4-D+0.2 mg1-1 Kn. The highest percentage of root induction was 80.0% and the highest number of roots per shoot was 5.0 observed in MS medium consisting of 2.0 mg1-1 NAA. Well rooted plantlets were acclimatized and successfully established onto the natural condition with 70% survival. Regenerated plantlets were morphologically uniform having normal leaf shape and growth. Download


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