Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

ISOLATION OF PLASMID- MEDIATED MULTIDRUG RESISTANT Escherichia coli FROM POULTRY (Pages : 46 - 50)

The study was conducted in Chittagong University, Bangladesh during January 2007 to April 2007 to investigate the relationship between the use of antibiotic and the development of resistance, 30 strains of Escherichia coli were isolated from fecal waste of chickens from Denm poultry farm, Chittagong, Bangladehs. E. coli strains were analyzed by agar disc diffusion to determine their susceptibility patterns to 7 antimicrobial agents. Plasmid DNA was isolated from ten isolates taken randomly by agarose-gel electrophoresis. Results of antibiotic susceptibility test showed that all of the isolates are multi-drug resistant (?4) and were resistant to Tetracycline (96.6%), Ciprofloxacin (30%), Penicillin (100%), Erythromycin (66.6%), Gentamycin (50%) and Chloramphenicol (100%) but all of them were sensitive to Imipenem. Turbidometric analysis of ciprofloxacin resistance pattern showed all of the isolates are highly resistant to ciprofloxacin even at concentration of 3000µg/ml. Agarose-gel electrophoresis of plasmid DNA from 10 isolates showed that all of them contain a high-molecular weight plasmid DNA. Our study highlight that the resistance development is directly related to use of antibiotics. the results suggest that the multi-drug resistant E.coli & plasmid containing multidrug resistant genes are present in the hospital waste may act as a possible source of transfer of these highly resistant pathogens and their genes to human that could be threat for the treatment of disease by commercially available antibiotics. Download