Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

MICROPOPAGATION OF STEVIA (Pages : 1 - 9)

The experiment was conducted at Biotechnology Laboratory, Bangladesh Sugarcane Research Institute (BSRI), Ishurdi, Pabna, during the period of July to December, 2005. In vitro propagation of Stevia was attempted by multiple shoot regeneration from the explants (shoot tips and nodal segments) followed by rooting of multiplied shoots. MS media in two different strengths (full MS and half MS) were used as culture media for shoot proliferation as well as root regeneration. Cytokinins viz. BAP and Kn were used for shoot multiplication in four concentrations (0, 0.5, 1.0, and 1.5 mg/l). Auxins viz. NAA and IBA were used for root induction of microcuttings in four concentrations (0, 0.5, 1.0, and 1.5 mg/l). Shoot tips showed better response for shoot proliferation than nodal segments. MS media in half and full strength were equally effective in shoot proliferation. BAP (1.0 mg/l) was superior to all other hormonal treatments for shoot proliferation. Full MS was superior to half MS. Full MS supplemented with NAA (1.5 mg/l) was the best medium for rooting of microcuttings. Download