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INFLUENCE OF GROWTH REGULATORS ON CALLUS INDUCTION FROM HYPOCOTYLS OF MEDIUM STAPLE COTTON (Gossypium hirsutum L). CULTIVAR BARAC B -67 (Pages : 17 - 22)

E. ABDELLATEF AND M. M. KHALAFALLAH

This study was carried out at laboratory of Plant tissue culture, Commission for Biotechnology and Genetic Engineering, Khartoum, Sudan during the period of April 2007 to February 2008 to develop a protocol for callus induction of elite Sudanese cotton. Callus cultures were initiated from hypocotyl explants of elite Sudanese medium staple cotton (Gossypium hirsutum L.) cultivar Barac B-67 on Gamborg's B5 basal media. Different types and concentrations of growth regulators were tested in order to obtain the best callus formation. Four auxin types, indole -3- acetic acid (IAA), ?-naphthalene acetic acid (NAA), indole-3- butyric acid (IBA), 2,4-dichloro-phenoxyacetic acid (2,4-D) at five concentrations(0.1, 0.2, 0.5,1.0 and 1.5 mgL-1 ) and two cytokinines, benzyl adenine (BA) and 6-furfuryl amino purine (Kin) at four levels (0.1, 0.5,1.0 and 1.5 mgL-1 ) in combination with NAA at (1.0 and 1.5 mgL-1 ) were used in this study. It was found that growth regulator type and concentration had a significant effect on the callus induction, the increment of callus index and callus physical appearance. The highest frequencies of callus growth index (8.13 and 8.0) were observed on hypoctyle explants cultured on B5 basal medium supplemented with 1.0 mgl -1 NAA in combination with 0.1 mgl -1 of Kin or BA, respectively. Medium containing Kin resulted in the formation of compact callus with large numbers of roots emerging from it. There was no callus formation on B5 basal medium. The callus induced on B5 medium containing 2, 4-D was brown in color and of low quality compared to that produced on B5 media containing NAA. Download


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