Papers

MOLECULAR MARKER BASED GENETIC DIVERSITY ANALYSIS IN AROMATIC RICE GENOTYPES USING SSR AND RAPD MARKERS (Pages : 23 - 34)

K. KIBRIA, F. NUR, S. N. BEGUM, M. M. ISLAM, S. K. PAUL, K. S. RAHMAN, AND S. M. M. AZAM

The study was undertaken to assess the genetic diversity among aromatic rice genotypes using simple sequence repeat (SSR) and randomly amplified polymorphic DNA (RAPD) markers through marker aided selection (MAS). The research work was performed during July 2006 to April 2008 at Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh, Bangladesh. Three SSR primers (RM223, RM342A and RM515) exhibited fourty six bands among the genotypes, while the average number of effective allele ranged from 1.78 to 2.49. The marker RM223 showed the highest polymorphism (66.67%). Out of 15 tested RAPD primers, 3 markers (OPA-02, OPA-10 and 67AB10G7) produced 34 distinct bands of which 32 were polymorphic. OPA02 and 67AB10G7 primers performed 100% polymorphism. Based on Nei’s genetic distance using the unweighted pair-group method with arithmetic means (UPGMA) dendrogram the SSR primers showed the highest genetic distance which was 2.306 and in case of RAPD marker it was 0.7634. Genotype pair (43-28-5-3-1 x 10-14-5-1-1) and (43-28-5-3-1 x 43-28-5- 2-1) were clustered in a separate cluster as they have diverse genetic background. Considering the genetic distance values the genotypes was genetically different from each other which could be used in breeding programme to have potential genetic gains. The results of the genetic diversity will be useful for the selection of the parents for developing rice breeding variety. Download


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