Papers

DEVELOPMENT OF A PCR BASED PROTOCOL FOR WSSV SCREENING FOR MAJOR CRUSTACEANS INHABITING IN CULTURED SHRIMP FARM (Pages : 9 - 17)

M. N. ISLAM, M. A. HOSSAIN AND M. N. AHSAN

The array of prevalence of white spot syndrome virus (WSSV) in cultured tiger shrimp, Bagda (Penaeus monodon) and non cultured Metapenaeus monoceros & crab, Scylla serrata from the coastal region of Bangladesh was determined by a two-step polymerase chain reactions (PCR)-based screening protocol using two set of oligonucleotide primers. The product of the first-step PCR of total genomic DNA isolated by a single-step DNA preparation without involving hazardous organic solvents from the muscle and hepatopancreas of sample from apparently diseased free pond and diseased pond served as the templates for the second-step PCR. The ability of identify a single piece of target DNA with high specificity, easiness, and cost-effectiveness of this method do not depend on using a patented WSSV detection kits as in elsewhere in the country. Up to date this is the first scientific report on applying PCR-based WSSV screening protocol in a diversity of species in Bangladesh. The ground-breaking works in Bangladesh and the results suggest that a PCR screening of WSSV infection for shrimp brood and larval stocks and other potential virus carriers could be an effective tool in battle against the fatal virus that has almost devastated the shrimp commerce of the country. Download


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