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MOLECULAR CHARACTERIZATION AND GENETIC DIVERSITY OF NERICA MUTANT RICE LINES USING SSR MARKER (Pages : 9 - 21)

M. NURUZZAMAN, L. HASSAN, S.N. BEGUM AND M.M. HUDA

Molecular characterization and genetic diversity analysis is important for the improvement of crop and help in the development of an appropriate breeding program. The present study was conducted to evaluate the molecular variation and genetic diversity of 31 NERICA mutant rice lines using 5 SSR markers. Among these 5 markers, 3 microsatellite markers showed clear polymorphism.A total of 39 alleles were detected among the 31 rice genotypes. The number of allele locus-1 ranged from 9 (RM32 and RM167) to 21(RM202) with an average of 13 allele locus-1. The Polymorphic information content (PIC) values ranged from 0.807 (RM32) to 0.940 (RM202) with an average of 0.854 locus-1. RM202 had highest genetic diversity (0.943), while RM32 had lowest genetic diversity (0.828) with a mean diversity of 0.868. On average, 226% of the 31 rice genotypes shared a common major allele ranging from 129% (RM202) to 323% (RM167) common allele at each locus. In this study, the larger range of similarity values for genotypes revealed by microsatellite markers provides greater confidence for the assessments of genetic diversity and relationships, which can be used in future breeding programs.Download


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