Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

IN VITRO REGENERATION OF SUGARCANE THROUGH SOMATIC EMBRYOGENESIS (Pages : 39 - 43)

A simple and efficient method of plant regeneration from midrib-derived callus was established in sugarcane. Explant was cultured in MS medium supplemented with 2,4-D and BA callus induction. The highest callus induction was found in MS medium supplemented with 2 mgl-1 2,4-D and 0.2 mgl-1 BA, where 72% of explants produced callus. Two month calli were subcultured in to same medium which has shown different types and color. The highest frequency of shoot formation was obtained in MS medium supplemented with 1.0 mgl-1 BA and 0.5 mgl-1 NAA. Half strength MS supplemented with 3 mgl-1 NAA performed best for root induction. After acclimatization of in vitro grown plantlet, 85% were survived. Download