Green Global
Foundation   Journal
...an emerging window for scientific research and publication across the globe

MICROSATELLITE MAPPING OF THE SEMI-DWARF GENE Rht-Leeds USING BULK SEGREGATION ANALYSIS IN DURUM WHEAT (Pages : 18 - 22)

Many wheat breeding programs in the world utilize giberellic acid insensitive and sensitive semidwarf germplasm to improve productivity and lodging resistance. Leeds mutant (Rht-Leeds) one of the height reducing recessive GA3-sensitive mutant can reduce plant height without reducing or restricting coleoptile elongation in durum wheat. Bulk segregation analysis revealed that the marker Xbarc147 on chromosome 3BS showed distinct polymorphism between the two parents, between the two bulks, and between individual semi-dwarf and tall plants in the F2 population of Leeds mutant × LD222. Microsatellite mapping indicated that the Rht-Leeds allele was found between the SSR markers Xbarc102 and Xbarc147 on chromosome arm 3BS. The allelic relationship indicated that Rht-Leeds and Rht5 were located on the same locus. Rht-Leeds would be help to further improvement of GA3-sensitive semidwarf wheat.Download