Green Global
Foundation   Journal
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IN VITRO MICROPROPAGATION OF STRAWBERRY (Fragaria ananassa) (Pages : 11 - 16)

The experiment was conducted at the Tissue Culture Laboratory, Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during the period from February 2008 to December 2009 to investigate the effect of different concentrations of BAP on virus free plant regeneration, shoot multiplication and different concentrations of IBA on in vitro root formation of strawberry accession FA002. Number of shoots/explant was the highest of 29.0 for runner tip with 10.0 mg/l BAP and the controls were the least (3.0 for runner tip and 2.6 for shoot tip. The maximum leaves/culture (10.2) was produced for 10.0 mg/l BAP with runner tip while the minimum (3.6) for 0.0 mg/l BAP with shoot tip. The highest length (5.0 cm) was produced for 1.0 mg/l BAP with runner tip which was statistically identical to 0.5, 1.5, 2.0 and 2.5 mg/l BAP with runner tip (4.2 cm, 4.4 cm, 4.6 cm and 4.2 cm respectively) and also statistically identical to 1.0, 1.5 and 2.0 mg/l BAP with shoot tip (4.2, 4.04 and 4.24 cm, respectively). For root initiation half strength MS medium supplemented with different levels of IBA (0, 0.5, 1.0, 1.5 and 2.0 mg/l) were used. Root numbers varied with different concentrations of IBA and type of explants. Number of roots/explant was found maximum (19.0) at 1mg/l IBA with shoot tip which was statistically identical to 1.0 mg/l with runner tip explants. Download