IN VITRO EVALUATION OF Trichoderma spp. AGAINST Phytophthora nicotianae (Pages : 20 - 25)


The Blank sank disease of tobacco caused by Phytophthora nicotianae results in considerable loss of the crop. This lab study aimed to investigate antagonistic activity of Trichoderma spp isolates against P. nicotianae. Three isolates of Trichoderma harzianum and one of T. viridae were evaluated in vitro for their efficacy suppressing the growth of P. nicotianae. The results showed considerable antagonistic potential for the biocontrol of P. nicotianae isolate by used all of Trichoderma species. T. harzianum (0034H) was found highly inhibitory to P. nicotianae in dual culture followed by T. harzianum (0034M) and T. harzianum (0034W). T. viridae (0034S) showed least effective to inhibit the mycelial growth of P. nicotianae. Microscopical observations indicate that the inhibitory effect was caused by an interaction that took place in close contact with the host hypha, causing lysing, swelling and coiling mycelia resulting potentially reduced of mycelial growth of P. nicotianae over control treatment.Download

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